Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C124R | IGP synthases formed with Q123R mutant HisF subunit has no measurable activity with glutamine in vitro | Escherichia coli |
E46G | IGP synthases formed with E46G mutant HisF subunit shows 2800fold reduction in the kcat/Km ratio for glutamine | Escherichia coli |
Q123R | IGP synthases formed with Q123R mutant HisF subunit has no measurable activity with glutamine in vitro | Escherichia coli |
R5H | IGP synthases formed with R5H mutant HisF subunit shows 1500fold reduction in the kcat/Km ratio for glutamine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
0.0175 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
0.0218 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
0.0426 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
1 | 1.2 | NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
1 | 4.5 | L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
5.4 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
7.4 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
13.4 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
14.3 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | Escherichia coli | - |
L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60595 and P60664 | P60595: IGP synthase glutamine amidotransferase subunit, P60664: IGP synthase cyclase subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | - |
Escherichia coli | L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | - |
Escherichia coli | D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
0.19 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
3.7 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
4.3 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
4.4 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme enzyme with a E46G mutant subunit HisF | Escherichia coli | |
5.2 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
5.2 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
5.3 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
5.7 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
5.7 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
0.024 | - |
L-glutamine | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
0.392 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
0.399 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
0.425 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli | |
0.581 | - |
NH4+ | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
120 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a R5H mutant subunit HisF | Escherichia coli | |
240 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a C124R mutant subunit HisF | Escherichia coli | |
300 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a E46G mutant subunit HisF | Escherichia coli | |
300 | - |
N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate | pH 8.0, 30°C, IGP synthase enzyme with a Q123R mutant subunit HisF | Escherichia coli |